This study investigated the potential hepatoprotective effect of oligoribonucleotides-D\n-mannitol complexes (ORNs-D-M) against thioacetamide (TAA)-induced hepatotoxicity in mice.\nThe hepatoprotective activity of ORNs-D-M was evaluated in thioacetamide (TAA)-treated C57BL/6J.\nResults indicate that treatment with ORNs-D-M displayed a protective effect at the TAA-induced\nliver injury. Treatment with ORNs-D-M, starting at 0 h after the administration of TAA, decreased\nTAA-elevated serum alanine aminotransferase (ALT) and \n-glutamyl transpeptidase (GGT). Activities\nof glutathione S-transferase (GST) and glutathione peroxidase (GPx), and levels of glutathione (GSH),\nwere enhanced with ORNs-D-M administration, while the hepatic oxidative biomarkers (TBA-reactive\nsubstances, protein carbonyl derivatives, protein-SH group) and myeloperoxidase (MPO) activity\nwere reduced. Furthermore, genetic analysis has shown that the ORNs-D-M decreases the expression\nof mRNA pro-inflammatory cytokines, such as tumor necrosis factor (TNF-) and interleukin-6\n(IL-6), profibrogenic cytokine-transforming growth factor 1 (TGF-1), as well as the principal protein\nof the extracellular matrixâ??collagen I. The present study demonstrates that ORNs-D-M exerts a\nprotective effect against TAA-induced liver injury, which may be associated with its anti-inflammatory\neffects, inhibition of overexpression of mRNA cytokines, and direct effects on the metabolism of\nthe toxin.
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